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61.
In this letter, a titanium aluminum carbide (Ti3AlC2) coated D-shaped fiber is proposed and demonstrated as a new saturable absorber (SA) for Q-switched laser pulse generation. In preparing the SA, the Ti3AlC2 powder is dispersed in liquid polyvinyl alcohol (PVA) before the solution is dropped and left to dry onto a polished surface of D-shape fiber. The SA is added to an erbium-doped fiber laser (EDFL) cavity to modulate the cavity loss for Q-switching. The Q-switched laser is obtained at 1 561 nm. The pulse width of the pulses can be varied between 7.4 µs and 5.1 µs with a corresponding repetition rate range from 41.26 kHz to 54.35 kHz, when the pump power is increased from 42.2 mW to 71.5 mW. At 71.5 mW pump, the pulse energy is obtained at 70.3 nJ. The signal-to-noise ratio (SNR) of the fundamental frequency is recorded at 67 dB, which indicates the stability of the laser.  相似文献   
62.
This article introduces a novel magnetic bead-based DNA extraction and purification device using active magnetic mixing approach. Mixing and separation steps are performed using functionalised superparamagnetic beads suspended in cell lysis buffer in a circular chamber that is sandwiched between two external magnetic coils. Non-uniform nature of magnetic field causes temporal and spatial distribution of beads within the chamber. This process efficiently mixes the lysis buffer and whole blood in order to extract DNA from target cells. Functionalized surface of the magnetic beads then attract the exposed DNA molecules. Finally, DNA-attached magnetic beads are attracted to the bottom of the chamber by activating the bottom magnetic coil. DNA molecules are extracted from magnetic beads by washing and re-suspension processes. In this study, a circular PMMA microchamber, 25 μL in volume, 500 μm in depth and 8 mm in diameter was fabricated to purify DNA from spiked bacterial cell cultures into the whole blood sample using Promega Magazorb DNA extraction kit. The lysis efficiency was evaluated using a panel of Gram-positive (Bacillus subtilis) and Gram-negative (Escherichia coli) bacterial cells cultures into the blood sample to achieve approximately 100,000 copy levels inside the chip. Manufacturer’s standard extraction protocol was modified to a more simplified process suitable for chip-based extraction. The lysis step was performed using 5 min incubation at 56 °C followed by 5 min incubation at room temperature for binding process. Temperature rise was generated and maintained by the same external magnetic coils used for active mixing. The yield/purity and recovery levels of the extracted DNA were evaluated using quantitative UV spectrophotometer and real-time PCR assay, respectively. Real-time PCR results indicated efficient chip-based bacterial DNA extraction using modified extraction protocol comparable to the standard bench-top extraction process.  相似文献   
63.
Most simulation models of electric machines use the coupled circuit approach, where the machine is considered as an electric circuit element with time-varying inductances (abc model) or with constant inductances (dq0 model). On the other hand, the rotating magnetic field approach, which considers the electric machine as two groups of windings producing rotating magnetic fields and can give insight into internal phenomena of the machines, has not yet received much attention in electric machines modeling, especially for machine transient analysis. Based on the rotating magnetic field approach, this paper presents a transient model of the induction machine including main flux saturation effect. Based on the direct computation of the magnetizing fluxes of all machine windings, the model represents instantaneous main flux saturation by simply introducing a main flux saturation factor. No iteration process is involved to incorporate the saturation effects. The model combines the advantages of the dq0 and abc models advantages, such as rapid computation time and nonsymmetrical conditions simulation, respectively. The simulation results and the experimental tests show advantages and verification of the model.  相似文献   
64.
The work presents simulations with the multirange Shan–Chen model developed by Sbragaglia et al. (2007) [18], which improved the Shan–Chen model for the proper surface tension term. Also, by introducing the matrix collision operator and extended equilibrium density distribution function, the density ratio is increased from 100 to 160. The Multi-Relaxation Time (MRT) method attracted the attention of researchers due to several advantages, such as better stability, simulations with Prandtl number different from unity, and possibilities to improve the accuracy of the scheme compared with BGK Single Time Relaxation model. Our recent results have shown that the combination of MRT methods with multiphase flow models can improve the achievable gas–liquid density ratio.  相似文献   
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The notion that ‘attitude drives behavior’ manifests itself in a variety of ways in educational and occupational settings. As applied to CAD competence development, industrial training of novice CAD users on their way to becoming competent CAD users consume a lot of corporate resources. This paper is the third paper in the line of research that attempts to answer the question having to do with what it takes to make a competent CAD user. Specifically, we examine the CAD-specific factors revolving around the trainees’ willingness-to-learn CAD.These factors are analyzed in two stages. At the start of the training, trainees’ initial attitude towards CAD is established by means of a short questionnaire. Afterwards, throughout the training, trainees’ behavior (online and offline practice) is gauged and, in turn, a relation is established to illustrate how this practice leads to the development of CAD-specific skills. For this purpose, another short questionnaire was utilized. Strong correlations were established relating the trainees’ CAD-specific behavior with the CAD-specific outcomes of learning CAD syntax.Furthermore, and in order to assess the quality of the trainees’ learning of CAD, overall competence was monitored throughout the study via performance measures that describe the time it took the trainees to build test models (speed), which reflects upon the ability to learn the syntax of the CAD tool (declarative knowledge). The sophistication of the models is also used as another measure. Correlating the trainees’ character attributes with these assessed measures, it was found that the stronger is the trainees’ will to learn CAD, the stronger is the likelihood to learn faster. Perhaps more importantly, trainees with initial favorable attitude toward CAD were shown to develop increasingly positive behavior that manifested through additional practice and other forms of visible effort.  相似文献   
68.
A novel Ni2+ optode was prepared by covalent immobilization of thionine, 3,7-diamine-5-phenothiazoniom thionineacetate, in a transparent agarose membrane. Influences of various experimental parameters on Ni2+ sensing, including the reaction time, the solution pH and the concentration of reagents were investigated. Under the optimized conditions, a linear response was obtained for Ni2+ concentrations ranging from 1.00 × 10−10 to 1.00 × 10−7 mol l−1 with an R2 value of 0.9985. The detection limit (3σ) of the method for Ni2+ was 9.30 × 10−11 mol l−1. The influence of several potentially interfering ions such as Ag+, Hg2+, Cd2+, Zn2+, Pb2+, Cu2+, Mn2+, Co3+, Cr3+, Al3+ and Fe3+ on the determination of Ni2+ was studied and no significant interference was observed. The membrane showed a good durability and short response time with no evidence of reagent leaching. The membrane was successfully applied for the determination of Ni2+ in environmental water samples.  相似文献   
69.
Understanding how learning occurs, and what improves or impedes the learning process is of importance to academicians and practitioners; however, empirical research on validating learning curves is sparse. This paper contributes to this line of research by collecting and analyzing CAD (computer-aided design) procedural and cognitive performance data for novice trainees during 16-weeks of training. The declarative performance is measured by time, and the procedural performance by the number of features used to construct a design part. These data were analyzed using declarative or procedural performance separately as predictors (univariate), or a combination of declarative or procedural predictors (multivariate). Furthermore, a method to separate the declarative and procedural components from learning curve data is suggested.  相似文献   
70.
A numerical study is presented for the two-dimensional flow of a micropolar fluid in a porous channel. The channel walls are of different permeability. The fluid motion is superimposed by the large injection at the two walls and is assumed to be steady, laminar and incompressible. The micropolar model due to Eringen is used to describe the working fluid. The governing equations of motion are reduced to a set of non-linear coupled ordinary differential equations (ODEs) in dimensionless form by using an extension of Berman’s similarity transformations. A numerical algorithm based on finite difference discretization is employed to solve these ODEs. The results obtained are further improved by Richardson’s extrapolation for higher order accuracy. Comparisons with the previously published work are performed and are found to be in a good agreement. It has been observed that the velocity and microrotation profiles change from the most asymmetric shape to the symmetric shape across the channel as the parameter R or the permeability parameter A are varied between their extreme values. The results indicate that larger the injection velocity at a wall relative to the other is, smaller will be the shear stress at it than that at the other. The position of viscous layer has been found to be more sensitive to the permeability parameter A than to the parameter R. The micropolar fluids reduce shear stress and increase couple stress at the walls as compared to the Newtonian fluids.  相似文献   
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